Peculiarities Of The Sorption Of Glucose Oxidase
PECULIARITIES OF THE SORPTION OF GLUCOSE OXIDASE
ON THE MESOPOROUS SUBSTANCES, OBTAINED
IN THE PRESENCE OF BIOTEMPLATES
Yu. G. Goltsov, Z. V. Smelaya, L. A. Matkovskaya, V. G. Il’in
L.V. Pisarzhevsky Institute of Physical Chemistry of NAS of Ukraine,
31 pr. Nauki, 03039 Kiev, Ukraine
The adsorption of the biologically active molecules into mesoporous molecular sieves has attracted great interest because it offers the combination of high selectivity of enzymatic reactions with the chemical and mechanical properties of these inorganic material. Aluminosilicate materials prepared in the presence of biotemplates are particular interest as appropriate supports for immobilszation of enzymes because of specific affinities to template.
It was established, that in the presence of various combination of lecithin and cetyltrimethylammonium bromide or octadecylamine as templating agents in aluminosilicate system mesoporous substances with a pore diameter up to 10 nm, and also biporous materials were obtained.
In the presence of glucose oxidase and cetyltrimethylammonium bromide combinations aluminosilicate substances with complex structure are formed (pore size distributions exibited 3 peaks, corresponding 3 effective size of mesopores in the 3-10 nm range).
Investigations of sorption of glucose oxidase into obtained substances indicate that the aluminosilicate mesoporous materials synthesized in the presence of biotemplates possess high sorption capacity.
The inclusion compounds on the base of mesoporous substances with large pores and great adsorption volume (up to 1.5 cm3/g) have the biggest content of glucose oxidase (near 100 mg/g). The sorption of glucose oxidase occurs within the pores of effective diameter more than 8 nm and results in essential decrease (reduction) of large pore adsorption volume. Porous structure of such inclusion compounds is characterized by a narrow distributions of pore sizes with effective diameter near 5 nm.
The inclusion compounds on the base of mesoporous substances with complex structure and small adsorption volume of more than 8.5 nm diameter
pores possess broad distribution of pore sizes and smaller content of glucose oxidase (up to 70 mg/g).
So the results obtained allow us to consider such materials as prospect for create high capable and selective sorbents for biomolecular sorption as well as active elements of chemical and biosensors.
Related articles::