Thin Layer Chromatographic
THIN LAYER CHROMATOGRAPHIC
AND SPECTROPHOTOMETRIC INVESTIGATIONS
OF PHENOLIC ACIDS
Z. Fekner1, G. Wronski1, A. Persona1, H.D. Smolarz2
1Department of Analitycal Chemistry, Maria Curie-Skłodowska University, Maria Curie-Skłodowska sq. 3, 20-031 Lublin, Poland
2 Department of Pharmaceutical Botany, Medical University,
Staszica st 4., 20-081 Lublin, Poland
Plant phenolic compounds, flavonoids, stilbenes, coumarins, phenolic acids are compounds possessing physiological and pharmacological pro-perties. They are present in our diet as components of fruits, vegetables and corn products.
Hence, a careful estimation of their content and determination of the correlation between their biological activity and hydrophobic ability becomes crucial. From the definition, the notion of hydrophobia refes to the phenomenon of the interaction between molecules of water and apolar groups of organic compounds. This kind of interaction is especially important in biological processes since it determines the transport of biologically active substances through biological membrane. Observing the tendency of molecules of organic compounds to pass from water solution through apolar organic layer we can determine the hydrophobic character of organic substances. The classical parameter used to this end is the partition coefficient logharytm (log P) of a given substance between n-octanol and water.
The aim of this paper was the determination of the values of the lipo-philicity parameter (log P) for the congomeric series of phenolic acids by use of spectrophotometric and chromatographic method.
The values of log P determined by chromatographic methods were cal-culated on the basis of experimentally determined values of RF by use of cali-bration curve.
The retention behaviour of phenolic derivatives of benzoic and cinnamic acids was examined on glass plates precoated with silica gel Si 60 with the
fluores- cence indicator F254 (E. Merck, Germany) and cellulose precoated plates (E. Merck Germany) using different mobile phases. The values of log P were determined on the basis of time measurement.
The results obtained by use of the above method were in accordance with the above mentioned. Experimental values were comparable with theoreticale calculations by use of the Crippen, Viswanadhan and Broto fragmentation.
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